Identification of Mycobacteria

Molecular Identification of Rare Clinical Mycobacteria by Application of 16S-23S Spacer Region Sequencing

Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...

Reliable urease test for identification of mycobacteria.

A simple, modified formation for urea broth gave consistent, reliable results to aid in the differentiation of Mycobacterium species. In a study of 1,346 isolates representing 17 different species, tests read after 7 days were distinct and reproducible. The use of this test facilitates the identification of Mycobacterium species, such as M. scrofulaceum, the M. avium complex, the M. terrae comp...

Simple amidase test for identification of mycobacteria.

A modified amidase test for differentiation of mycobacteria is described. A total of 224 atypical mycobacteria, 154 Mycobacterium tuberculosis, and 26 M. bovis strains were classified by this procedure. Of the 404 strains of various species studied, 400 exhibited an amidase spectrum identical to the established pattern. The simplicity of this method may promote its application in routine examin...

Molecular Detection and Identification of Mycobacteria

Identification of mycobacteria by using INNO LiPA.

I have read with interest the recent paper of Suffys et al. (1) concerning the identification of 157 mycobacterium strains by using the INNO LiPA Mycobacteria (LiPA) assay. The same journal had published, 1 year ago, a similar paper, which I coauthored, on 238 strains (2), the results of which are now substantially confirmed. I feel, however, that Suffys et al. did not take it into due account;...